Miniaturized and Portable Electrochemiluminescence Biosening Platform with Laser-Induced Graphene based Electrodes
Sanket Goel, Senior Member, IEEE
MEMS, Microfluidics and Nanoelectronics (MMNE) Lab, Department of Electrical and Electronics Engineering, Birla Institute of Technology and Science (BITS) Pilani, Hyderabad Campus, Hyderabad, India 500078
sgoel@hyderabad.bits-pilani.ac.in
Abstract
The rising need of miniaturized point-of-care (POC) devices has led to the cost-effective solutions due to their simple operation and fast response. For such devices, amongst various detection mechanisms, Electrochemiluminescence (ECL), a combination of electrochemistry (EC) and chemiluminescence (CL), has proven to be a highly sensitive method providing excellent specificity, cost-efficiency and flexibility. Such ECL systems have wide range of applications such as environmental monitoring, food control and biomedical diagnostics. With this impetus, herein, CO2 Laser-Induced Graphene (LIG) based ECL system, integrated with Bipolar Electrode (BPE) and Single Electrode (SE), has been developed. Further, its application for enzymeless sensing of various biomarkers, such as H2O2, Glucose (G), Xanthine (X), Dopamine (D) and vitamin B12, has been validated. Low-cost and easily available flexible polyimide (PI) sheet has been effectively used for the fabrication of such device. The electrodes were fabricated on PI substrate by creating optimized LIG in a single-step. With optimized speed and power of CO2 Laser, non-conducting portion of PI gets converted into conducting zone (electrodes) for ECL imaging. A 3D printed miniaturized portable system was developed to detect and monitor the ECL signals. Further, bulky external power supply was replaced by android smartphone, which was used to not only supply power to ECL sensor through DC to DC buck boost converter but also to capture the ECL images. With miniaturized portable 3D printed platform, sensing of these biomarkers was accomplished by different sets of ECL platforms for the extended linear range. Excellent limit of detection (LOD) was measured for different biomarkers beyond the benchmarked values. Therefore, developed 3D printed portable miniaturized ECL platform can be used in broad areas such as food control, biomedical applications and in point of care testing (POCT) systems.
Short Bio
Sanket Goel headed the Department of Electrical and Electronics Engineering Department at BITS-Pilani (2017-2020), and R&D department at the University of Petroleum & Energy Studies (UPES) (2011-2015). Sanket did his BSc (H- Physics) from Ramjas College, Delhi University; MSc (Physics) from IIT Delhi; PhD (Electrical and Computer Engineering) from University of Alberta, Canada in 1998, 2000, and 2006 respectively. He has worked with Institute of Plasma Research, Gandhinagar (2000-2001) and DEBEL-DRDO, Bangalore (2006). Sanket did his postdoc at Stanford University (2006-2008) and was a PI with ASTAR, Singapore (2008- 20011). His lab focusses on MEMS, Microfluidics and Nanoelectronics for Energy and Bio Applications, where he has been implementing several Indian and overseas funded projects. Sanket has won several awards, like Fulbright fellowship (2015), American Electrochemical Society’s Best students paper award (2005) and University of Alberta PhD thesis award (2005). Sanket has >180 publications and 12 patents to his credits, and has delivered >70 invited talks and guided/guiding 25 PhD students. He is an Associate Editor of IEEE Transactions on NanoBioscience, IEEE Sensors Journal, IEEE Access and Applied Nanoscience. He is also a Visiting Associate Professor with UiT, The Arctic University of Norway.