Single nanoparticle Impact electrochemistry for highly sensitive cancer protein detection in human serum
Yi-Ge Zhou
Institute of Chemical Biology and Nanomedicine, State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China
yigezhou@hnu.edu.cn
Abstract
Protein quantification with high throughput and high sensitivity is essential in the early diagnosis and elucidation of molecular mechanisms for many diseases. Conventional approaches for protein assay often suffer from high costs, long analysis time and insufficient sensitivity. The recently emerged nano-impact electrochemistry (NIE), as a contrast, allows in situ detection of analytes one at a time with simplicity, fast response, high throughput and the potential of reducing the detection limit down to single entity level. This talk will present a NIE-enabled electrochemical immunoassay using silver nanoparticles (AgNPs) as probes for the detection of CYFRA21-1, a typical protein marker for lung carcinoma. This strategy is based on the measurement of the impact frequency and the charge intensity of the electrochemical oxidation of individual AgNPs before and after they are modified with anti-CYFRA21-1 and in turn immunocomplexed with CYFRA21-1. Both the frequency and intensity modes…
Short Bio
Prof. Yi-Ge Zhou received her B.S. and M.S. degrees in Chemistry from Nanjing University (China). She then continued her study at the University of Oxford and obtained her PhD degree in Chemistry in 2013. After that, she worked as a postdoctoral fellow at University of Toronto and Northwestern University (USA). She is currently a professor at Hunan University (China). Her research focuses on single nanoparticle electrochemistry and dip-pen nanolithography based nanofabrication.