Multifunctional Nanoparticle for Cancer Theranostics and Drug Delivery
Ren-Jei Chung
Department of Chemical Engineering and Biotechnology, National Taipei University of Technology (Taipei Tech), Taiwan, Taipei, 10608
rjchung@mail.ntut.edu.tw
Abstract
Gliomas display a poor disease prognosis causing death within 15 months after diagnosis. Chemotherapy has offered some hope to target this, however, it is majorly ineffective due to the low therapeutic window, poor efficacy and high cytotoxicity. To overcome these challenges, we conjugated Angiopep-2, a cell penetrating peptide (CPP) to Iron Gold (Fe-Au) alloy nanoparticles and investigated the ability of Ang-Fe-Au Nps conjugate to limit glioma growth via magnetic field induced hyperthermia. Our results show that 6.44nm sized conjugated Fe-Au Nps were superparamagnetic, enhanced negative Glioma image contrast and displayed a 12°C temperature elevation when magnetically stimulated, indicating applications in medical imaging and hyperthermia-based therapy. Angiopep-2 conjugation resulted in 1.5-fold higher ingestion by C6 glioma cells than L929 fibroblasts, indicating specific glioma targeting and resulting in 90% decrement in cell viability due to magnetic field induced hyperthermia. Immunohistochemical analysis showed an enhanced coagulative necrosis, glial fibrillary acidic protein (GFAP) expression and decreased Ki67 labelling index in rat treated with Ang-Fe-Au Nps which translated to a 5-fold decrement in tumor volume, consequently resulting in an increased survival time by 7 days. The dual application of this platform opens new doors towards cancer theranostics with minimal invasiveness.
Short Bio
Dr. Ren-Jei Chung is Professor in the Department of Chemical Engineering and Biotechnology at National Taipei University of Technology (Taipei Tech), and also serves as the Director in the Advanced Materials Research Center. He has his research interests in Biomaterials, Biomedical Applications of Nanotechnology, Tissue Engineering and Biosensors. He has been awarded for Young Investigator Award from 2014 International Symposium of Materials on Regenerative Medicine; Dr. Shechtman Young Researcher Award from Taipei Tech in 2015; 2017 Excellent Young Member of Taiwan Ceramic Society; 2017 Excellent Junior Research Investigators Award from Association of Chemical Sensors in Taiwan; 2018 Excellent Research Award from Taipei Tech College of Engineering; 2019 Excellent Research Award from Taipei Tech; 2019 Young Scholar Award from Taiwan Association for Coatings and Thin Film Technology; and granted for 2019 Project for Excellent Junior Research Investigators from Ministry of Science and Technology (MOST Taiwan).